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PRODUCT CODE: ET1702-91

Recombinant ARF6 Monoclonal Antibody (ET1702-91)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.
  • Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.
  • ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ARF6 Monoclonal Antibody (ET1702-91)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, HepG2, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF101-2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

P62330

PROTEIN NAME

ARF6

SYNONYMS

ADP ribosylation factor 6 antibody; ADP ribosylation factor protein 6 antibody; ADP-ribosylation factor 6 antibody; ARF6 antibody; ARF6_HUMAN antibody; DKFZp564M0264 antibody; Small GTP binding protein antibody; Small GTPase antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Arf family.

TISSUE SPECIFICITY

Ubiquitous, with higher levels in heart, substantia nigra, and kidney.

SUBCELLULAR LOCATION

Golgi apparatus, Cell membrane, Endosome membrane, Cell projection, Midbody, Cytoplasm, Cleavage furrow.

FUNCTION

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of Actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein.