PRODUCT CODE: ET1612-12

Recombinant ARF1 Monoclonal Antibody (ET1612-12)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.
  • Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARF1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ARF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ARF1 Monoclonal Antibody (ET1612-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, human colon cancer tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2002

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ADP-ribosylation factor 1

GENE NAME

ARF1

SYNONYMS

ARF1

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Arf family.

POST-TRANSLATIONAL MODIFICATION

Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.

SUBCELLULAR LOCATION

Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic density. Membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor.

FUNCTION

GTP-binding protein involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).; (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.