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PRODUCT CODE: ET7107-47

Recombinant Apolipoprotein A II Monoclonal Antibody (ET7107-47)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

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ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apolipoprotein A II in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Apolipoprotein A II Monoclonal Antibody (ET7107-47)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, LOVO, SH-SY-5Y, rat big intestine tissue, rat liver tissue, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB70-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P02652

PROTEIN NAME

Apolipoprotein A II

SYNONYMS

APO A2 antibody; Apo AII antibody; Apo-AII antibody; APOA 2 antibody; ApoA II antibody; ApoA-II antibody; APOA2 antibody; APOA2_HUMAN antibody; APOAII antibody; Apolipoprotein A II antibody; Apolipoprotein A-II(1-76) antibody; Apolipoprotein A2 antibody; Apolipoprotein AII antibody; ApolipoproteinA II antibody; OTTHUMP00000032244 antibody

SEQUENCE SIMILARITIES

Belongs to the apolipoprotein A2 family.

TISSUE SPECIFICITY

Plasma; synthesized in the liver and intestine.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation sites are present in the extracellular medium.; Apolipoprotein A-II is O-glycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

s are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a co-factor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesterylesters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.