Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Apolipoprotein A II Monoclonal Antibody (ET7107-47)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
HepG2, LOVO, SH-SY-5Y, rat big intestine tissue, rat liver tissue, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB70-35
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
11 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
ICC
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Apolipoprotein A II
SYNONYMS
APO A2 antibody; Apo AII antibody; Apo-AII antibody; APOA 2 antibody; ApoA II antibody; ApoA-II antibody; APOA2 antibody; APOA2_HUMAN antibody; APOAII antibody; Apolipoprotein A II antibody; Apolipoprotein A-II(1-76) antibody; Apolipoprotein A2 antibody; Apolipoprotein AII antibody; ApolipoproteinA II antibody; OTTHUMP00000032244 antibody
SEQUENCE SIMILARITIES
Belongs to the apolipoprotein A2 family.
TISSUE SPECIFICITY
Plasma; synthesized in the liver and intestine.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation sites are present in the extracellular medium.; Apolipoprotein A-II is O-glycosylated.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
s are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a co-factor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesterylesters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.