PRODUCT CODE: ET1612-86

Recombinant APC6 Monoclonal Antibody (ET1612-86)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
  • Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
  • ICC staining APC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC6 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant APC6 Monoclonal Antibody (ET1612-86)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

RH-35, MCF-7, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD085-8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cell division cycle protein 16 homolog

GENE NAME

CDC16

SYNONYMS

APC6, CDC16Hs, CDC16

SEQUENCE SIMILARITIES

Belongs to the APC6/CDC16 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.

FUNCTION

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.