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PRODUCT CODE: ET1612-86

Recombinant APC6 Monoclonal Antibody (ET1612-86)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
  • Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
  • ICC staining APC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APC6 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant APC6 Monoclonal Antibody (ET1612-86)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

RH-35, MCF-7, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD085-8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

Q13042

PROTEIN NAME

APC6

SYNONYMS

ANAPC6 antibody; Anaphase promoting complex subunit 6 antibody; Anaphase-promoting complex subunit 6 antibody; Apc 6 antibody; APC6 antibody; CDC 16 antibody; CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody; Cdc16 antibody; CDC16 homolog antibody; CDC16 protein antibody; CDC16_HUMAN antibody; CDC16Hs antibody; Cell division cycle 16 antibody; Cell division cycle 16 homolog antibody; Cell division cycle protein 16 homolog antibody; CUT9 antibody; Cyclosome subunit 6 antibody

SEQUENCE SIMILARITIES

Belongs to the APC6/CDC16 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.