Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant AP2M1 Monoclonal Antibody (ET1612-33)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
PC-3M cell lysates, HepG2, SH-SY5Y, MCF-7, human kidney tissue, mouse brain tissue, PANC-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD0746
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
50 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
AP2M1
SYNONYMS
Adapter-related protein complex 2 mu subunit antibody; Adaptin mu 1 antibody; Adaptin-mu2 antibody; Adaptor protein complex AP 2 subunit mu antibody; Adaptor protein complex AP-2 subunit mu antibody; Adaptor related protein complex 2 mu 1 subunit antibody; AP 2 mu 2 chain antibody; AP-2 complex subunit mu antibody; AP-2 mu chain antibody; Ap2m1 antibody; AP2M1_HUMAN antibody; AP50 antibody; CLAPM1 antibody; Clathrin adaptor complex AP2 mu subunit antibody; Clathrin assembly protein complex 2 medium chain antibody; Clathrin associated/assembly/adaptor protein medium 1 antibody; Clathrin coat adaptor protein AP50 antibody; Clathrin coat assembly protein AP50 antibody; Clathrin coat associated protein AP50 antibody; Clathrin coat-associated protein AP50 antibody; HA2 50 kDa subunit antibody; mu2 antibody; Plasma membrane adaptor AP-2 50 kDa protein antibody
SEQUENCE SIMILARITIES
Belongs to the adaptor complexes medium subunit family.
TISSUE SPECIFICITY
Expressed in the brain (at protein level).
SUBCELLULAR LOCATION
Cell membrane, coated pit.
FUNCTION
Adaptins are heterotetrameric subunits of adaptors, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis. Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles. The Adaptin family, comprising a, b, b' and g classes, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes. Two main types of adaptor proteins (APs), AP-1 and AP-2, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, respectively. Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1). AP-2m1, a 435 amino acid protein, links Clathrin to receptors in coated vesicles.