PRODUCT CODE: ET1702-62

Recombinant Annexin V Monoclonal Antibody (ET1702-62)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: JAR
  • Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: JAR
  • ICC staining Annexin V in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Annexin V in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with Annexin V antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: JAR

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Annexin V Monoclonal Antibody (ET1702-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

JAR, A431, Hela, HepG2, human liver tissue, mouse liver tissue, mouse brain tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF50-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Annexin A5

GENE NAME

ANXA5

SYNONYMS

CBP-I, PP4, PAP-I, VAC-alpha, ANXA5

SEQUENCE SIMILARITIES

Belongs to the annexin family.

POST-TRANSLATIONAL MODIFICATION

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

FUNCTION

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.