PRODUCT CODE: ET1612-56

Recombinant AMPK beta 1 Monoclonal Antibody (ET1612-56)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution.
  • Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution.
  • Flow cytometric analysis of A431 cells with AMPK beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant AMPK beta 1 Monoclonal Antibody (ET1612-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD082-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

5'-AMP-activated protein kinase subunit beta-1

GENE NAME

PRKAB1

SYNONYMS

AMPK subunit beta-1, AMPKb, PRKAB1

SEQUENCE SIMILARITIES

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

FUNCTION

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).