PRODUCT CODE: ET1705-31

Recombinant alpha Tubulin 4A Monoclonal Antibody (ET1705-31)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: A431 <br />
Lane 2: Rat brain tissue <br />
Lane 3: NIH-3T3 <br />
Lane 4: PC-12
  • Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: A431 <br />
Lane 2: Rat brain tissue <br />
Lane 3: NIH-3T3 <br />
Lane 4: PC-12
  • ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: Rat brain tissue
Lane 3: NIH-3T3
Lane 4: PC-12

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant alpha Tubulin 4A Monoclonal Antibody (ET1705-31)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

WB:A431, NIH-3T3, PC-12, rat brain tissue;ICC: Hela, NIH-3T3, SW480; IHC: Human tonsil tissue, human thyroid tissue, mouse brain tissue, human placenta tissue, mouse testes tissue; FC: Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM73-24

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:100-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tubulin alpha-4A chain

GENE NAME

TUBA4A

SYNONYMS

TUBA4A, TUBA1

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.; Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton.

FUNCTION

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.