PRODUCT CODE: ET7107-31

Recombinant Alpha Synuclein Monoclonal Antibody (ET7107-31)

  • Recombinant
  • Test-Size

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Alpha Synuclein on human fetal brain tissue lysate using anti-Alpha Synuclein antibody at 1/500 dilution.
  • Western blot analysis of Alpha Synuclein on human fetal brain tissue lysate using anti-Alpha Synuclein antibody at 1/500 dilution.
  • ICC staining Alpha Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Alpha Synuclein antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Alpha Synuclein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Alpha Synuclein on human fetal brain tissue lysate using anti-Alpha Synuclein antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Alpha Synuclein Monoclonal Antibody (ET7107-31)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human brain tissue lysates, Hela, mouse brain tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB42-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

16 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Alpha Synuclein

SYNONYMS

Alpha synuclein antibody; Alpha-synuclein antibody; Alpha-synuclein, isoform NACP140 antibody; alphaSYN antibody; MGC105443 antibody; MGC110988 antibody; MGC127560 antibody; MGC64356 antibody; NACP antibody; Non A beta component of AD amyloid antibody; Non A4 component of amyloid antibody; Non A4 component of amyloid precursor antibody; Non-A beta component of AD amyloid antibody; Non-A-beta component of alzheimers disease amyloid , precursor of antibody; Non-A4 component of amyloid precursor antibody; Non-A4 component of amyloid, precursor of antibody; OTTHUMP00000218549 antibody; OTTHUMP00000218551 antibody; OTTHUMP00000218552 antibody; OTTHUMP00000218553 antibody; OTTHUMP00000218554 antibody; PARK 1 antibody; PARK 4 antibody; PARK1 antibody; PARK4 antibody; Parkinson disease (autosomal dominant, Lewy body) 4 antibody; Parkinson disease familial 1 antibody; SNCA antibody; Snca synuclein, alpha (non A4 component of amyloid precursor) antibody; SYN antibody; Synuclein alpha antibody; Synuclein alpha 140 antibody; Synuclein, alpha (non A4 component of amyloid precursor) antibody; SYUA_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the synuclein family.

TISSUE SPECIFICITY

Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.; Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.; Ubiquitinated. The predominant conjugate is the diubiquitinated form (By similarity).; Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

SUBCELLULAR LOCATION

Secreted, nucleus, cytoplasm, membrane, synapse.

FUNCTION

The synuclein family members, including α-synuclein (also designated NACP for non-β-Amyloid component) and β-synuclein, are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. α-synuclein is localized to neuronal cell bodies and synapses. α-synuclein was first identified as a component of Alzheimer's disease amyloid plaques. Abnormal platelet function in Alzheimer's disease has been demonstrated. During megakaryocytic differentiation α-synuclein has been found to be upregulated, while β-synuclein is downregulated, indicating that coordinate expression of synucleins may be important during hematopoetic cell differentiation. A mutant form of α-synuclein has been found in patients with early onset Parkinson's disease.