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PRODUCT CODE: ET1706-44

Recombinant Alpha Fodrin Monoclonal Antibody (ET1706-44)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Alpha Fodrin on different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Hela<br /> Lane 2: Jurkat<br /> Lane 3: NIH-3T3<br /> Lane 4:Rat brain<br /> Lane 5:Mouse brain<br />
  • Western blot analysis of Alpha Fodrin on different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: Hela<br /> Lane 2: Jurkat<br /> Lane 3: NIH-3T3<br /> Lane 4:Rat brain<br /> Lane 5:Mouse brain<br />
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
Western blot analysis of Alpha Fodrin on different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: NIH-3T3
Lane 4:Rat brain
Lane 5:Mouse brain

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Alpha Fodrin Monoclonal Antibody (ET1706-44)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, NIH-3T3, Jurkat, rat brain tissue lysate, mouse brain tissue, human liver cancer tissue, human pancreas tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU32-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

285/150 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q13813

PROTEIN NAME

Spectrin alpha chain, non-erythrocytic 1

GENE NAME

SPTAN1

SYNONYMS

NEAS, SPTA2

SEQUENCE SIMILARITIES

Belongs to the spectrin family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.

FUNCTION

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.