PRODUCT CODE: ET1702-30

Recombinant Alpha Actinin 2 Monoclonal Antibody (ET1702-30)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.
  • Western blot analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ACTN2 antibody. Counter stained with hematoxylin.
Western blot analysis of ACTN2 on human heart lysates using anti-ACTN2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Alpha Actinin 2 Monoclonal Antibody (ET1702-30)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human heart tissue, rat skeletal muscle tissue, rat heart tissue, mouse skeletal tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0980

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

104 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Alpha Actinin 2

SEQUENCE SIMILARITIES

Belongs to the alpha-actinin family.

TISSUE SPECIFICITY

Expressed in both skeletal and cardiac muscle.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by FBXL22, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.