PRODUCT CODE: ET1610-46

Recombinant alpha 1 Catenin Monoclonal Antibody (ET1610-46)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of alpha 1 Catenin on different lysates using anti-alpha 1 Catenin antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: A431
  • Western blot analysis of alpha 1 Catenin on different lysates using anti-alpha 1 Catenin antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: A431
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha 1 Catenin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-alpha 1 Catenin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-alpha 1 Catenin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with alpha 1 Catenin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of alpha 1 Catenin on different lysates using anti-alpha 1 Catenin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant alpha 1 Catenin Monoclonal Antibody (ET1610-46)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, HepG2, mouse colon tissue, mouse prostate tissue, mouse uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC0619

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Catenin alpha-1

GENE NAME

CTNNA1

SYNONYMS

CTNNA1

SEQUENCE SIMILARITIES

Belongs to the vinculin/alpha-catenin family.

TISSUE SPECIFICITY

Expressed ubiquitously in normal tissues.

POST-TRANSLATIONAL MODIFICATION

Sumoylated.; Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.

SUBCELLULAR LOCATION

[Isoform 1]: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries.; [Isoform 3]: Cell membrane; Peripheral membrane protein; Cytoplasmic side.

FUNCTION

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.