PRODUCT CODE: ET1705-74

Recombinant ALIX Monoclonal Antibody (ET1705-74)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela Lane 2: PC-12 Lane 3: NIH-3T3
  • Western blot analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela Lane 2: PC-12 Lane 3: NIH-3T3
  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ALIX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ALIX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ALIX antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with ALIX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of ALIX on different cell lysates using anti-ALIX antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela Lane 2: PC-12 Lane 3: NIH-3T3

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ALIX Monoclonal Antibody (ET1705-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC-12, NIH-3T3, rat stomach tissue, human colon cancer tissue, human prostate tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM85-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Programmed cell death 6-interacting protein

GENE NAME

PDCD6IP

SYNONYMS

PDCD6-interacting protein, PDCD6IP, AIP1, ALIX, KIAA1375

POST-TRANSLATIONAL MODIFICATION

May be phosphorylated on tyrosine residues by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted, extracellular exosome. Cell junction, tight junction. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 at centrosomes of non-dividing cells. Component of the actomyosin-tight junction complex (By similarity). PDCD6IP targeting to the midbody requires the interaction with CEP55.

FUNCTION

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP. By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).; (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).