PRODUCT CODE: ET1705-91

Recombinant Aldolase Monoclonal Antibody (ET1705-91)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Hela <br />
  Lane 3: Mouse liver <br />
  Lane 4: Rat liver<br />
  Lane 5: Rat spleen
  • Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 2: Hela <br />
  Lane 3: Mouse liver <br />
  Lane 4: Rat liver<br />
  Lane 5: Rat spleen
  • ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
  • Western blot analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution.
Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: Mouse liver
Lane 4: Rat liver
Lane 5: Rat spleen

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Aldolase Monoclonal Antibody (ET1705-91)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, A549, mouse liver,rat liver, rat spleen tissue lysate, Zebrafish tissue lysates, A431, SK-Br-3, rat skeletal muscle tissue, human fetal skeletal muscle tissue, human pancreas tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM54-19

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Aldolase

SYNONYMS

ALDA antibody; Aldo1 antibody; ALDOA antibody; ALDOA_HUMAN antibody; Aldolase 1 antibody; Aldolase A antibody; Aldolase A fructose bisphosphatase antibody; Aldolase A fructose bisphosphate antibody; Aldolase, fructose-bisphosphate A antibody; Epididymis secretory sperm binding protein Li 87p antibody; FRUCTOALDOLASE A antibody; Fructose 1 6 bisphosphate triosephosphate lyase antibody; Fructose bisphosphate aldolase A antibody; Fructose bisphosphate aldolase antibody; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody; Fructose-bisphosphate aldolase A antibody; Fructose-bisphosphate aldolase A Muscle-type antibody; GSD12 antibody; HEL S 87p antibody; Lung cancer antigen NY LU 1 antibody; Lung cancer antigen NY-LU-1 antibody; MGC10942 antibody; MGC17716 antibody; MGC17767 antibody; Muscle type aldolase antibody; Muscle-type aldolase antibody; RNALDOG5 antibody

SEQUENCE SIMILARITIES

Belongs to the class I fructose-bisphosphate aldolase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A; the liver-specific Aldolase B; and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldo-lase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.