PRODUCT CODE: ET1601-26

Recombinant Alas1 Monoclonal Antibody (ET1601-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: JAR <br />
Lane 2: HUVEC <br />
Lane 3: Hela
  • Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: JAR <br />
Lane 2: HUVEC <br />
Lane 3: Hela
  • ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: JAR
Lane 2: HUVEC
Lane 3: Hela

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Alas1 Monoclonal Antibody (ET1601-26)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, MCF-7, Hela, HepG2, JAR.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SA70-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

5-aminolevulinate synthase, nonspecific, mitochondrial

GENE NAME

ALAS1

SYNONYMS

ALAS-H

SEQUENCE SIMILARITIES

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

SUBCELLULAR LOCATION

Mitochondrion matrix.