PRODUCT CODE: ET1704-57

Recombinant Aggrecan Monoclonal Antibody (ET1704-57)

  • Recombinant

Applications

  • IHC-P

REACTIVITY

  • Human

  • Rat

Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Aggrecan Monoclonal Antibody (ET1704-57)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat cartilage tissue, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA40-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

250/246/239 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Aggrecan core protein

GENE NAME

ACAN

SYNONYMS

CSPCP, Chondroitin sulfate proteoglycan 1, ACAN, AGC1, CSPG1, MSK16

SEQUENCE SIMILARITIES

Belongs to the aggrecan/versican proteoglycan family.

TISSUE SPECIFICITY

Restricted to cartilages.

DEVELOPMENTAL STAGE

Expression was detected in chondrocytes throughout the developing skeleton.

POST-TRANSLATIONAL MODIFICATION

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix.

FUNCTION

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.