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PRODUCT CODE: ET1704-17

Recombinant ADFP Monoclonal Antibody (ET1704-17)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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ICC staining ADFP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ADFP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ADFP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ADFP in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with ADFP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining ADFP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ADFP Monoclonal Antibody (ET1704-17)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, JAR, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA31-81

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q99541

PROTEIN NAME

ADFP

SYNONYMS

ADFP antibody; Adipophilin antibody; Adipose differentiation related protein antibody; Adipose differentiation-related protein antibody; ADRP antibody; MGC10598 antibody; Perilipin-2 antibody; PLIN2 antibody; PLIN2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the perilipin family.

TISSUE SPECIFICITY

Milk lipid globules.

POST-TRANSLATIONAL MODIFICATION

Acylated; primarily with C14, C16 and C18 fatty acids.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

May be involved in development and maintenance of adipose tissue.