PRODUCT CODE: ET7106-60

Recombinant ACTL6A Monoclonal Antibody (ET7106-60)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.
  • Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.
  • ICC staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ACTL6A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.
Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ACTL6A Monoclonal Antibody (ET7106-60)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, mouse brain tissue, LOVO, PC-3M, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU40-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:100

  • IHC-P

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

ACTL6A

SYNONYMS

53 kDa BRG1 associated factor A antibody; 53 kDa BRG1-associated factor A antibody; ACL6A_HUMAN antibody; Actin like 6A antibody; Actin like protein 6A antibody; Actin related protein 4 antibody; Actin related protein antibody; Actin related protein Baf53a antibody; Actin-like protein 6A antibody; Actin-related protein Baf53a antibody; ACTL 6 antibody; ACTL 6A antibody; ACTL6 antibody; ACTL6A antibody; Arp4 antibody; ARPN BETA antibody; ArpNbeta antibody; BAF 53A antibody; BAF complex 53 kDa subunit antibody; BAF53 antibody; BAF53A antibody; BRG1 associated factor antibody; BRG1-associated factor 53A antibody; hArpN beta antibody; INO80 complex subunit K antibody; INO80K antibody; MGC5382 antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

ACTL6A also named BAF53. The SWI/SNF complex regulates gene expression via ATP-dependent chromatin remodeling. Brm (SNF2-a), Brg-1 (SNF2-b), Ini1 (integrase interactor 1, SNF5), BAF53 (ARPnβ), BAF57, BAF155 (SRG3), and BAF170 make up the functional core. BAF53 homologues from yeast to humans contain a conserved N-terminal motif, which contains residues at serine 2 and tyrosine 6, which play important roles in BAF53 activity. The BAF53 protein shuttles between the nucleus and cytoplasm. BAF53 also forms a complex with TIP49 and TIP48, which mediates c-Myc oncogenic activity.