PRODUCT CODE: ET1702-07

Recombinant ABL2 Monoclonal Antibody (ET1702-07)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ABL2 on different lysates using anti-ABL2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: PC-12 <br />
 Lane 3: Jurkat
  • Western blot analysis of ABL2 on different lysates using anti-ABL2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: PC-12 <br />
 Lane 3: Jurkat
  • Flow cytometric analysis of Raji cells with ABL2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of ABL2 on different lysates using anti-ABL2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: Jurkat

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ABL2 Monoclonal Antibody (ET1702-07)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, Jurakt, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0951

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

128 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine-protein kinase ABL2

GENE NAME

ABL2

SYNONYMS

ABL2

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).; Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton.

FUNCTION

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.