PRODUCT CODE: ET1702-40

Recombinant ABCG2 Monoclonal Antibody (ET1702-40)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Human placenta
  • Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Human placenta
  • ICC staining ABCG2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ABCG2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ABCG2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with ABCG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human placenta

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ABCG2 Monoclonal Antibody (ET1702-40)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, MCF-7, Hela, HepG2, human placenta tissue, mouse placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0994

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Broad substrate specificity ATP-binding cassette transporter ABCG2

GENE NAME

ABCG2

SYNONYMS

ABCG2

SEQUENCE SIMILARITIES

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

TISSUE SPECIFICITY

Highly expressed in placenta. Low expression in small intestine, liver and colon. Expressed in brain (at protein level).

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.; Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein; Multi-pass membrane protein; Multi-pass membrane protein.

FUNCTION

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk (By similarity). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity).