PRODUCT CODE: ET1611-63

Recombinant ABCF1 Monoclonal Antibody (ET1611-63)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.
  • Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.
  • ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ABCF1 Monoclonal Antibody (ET1611-63)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, PC-3M, rat stomach tissue, human colon cancer tissue, human liver cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN07-12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ABCF1

SYNONYMS

ABC27 antibody; ABC50 antibody; ABCF1 antibody; ABCF1_HUMAN antibody; ATP binding cassette 50 (TNF alpha stimulated) antibody; ATP binding cassette 50 antibody; ATP binding cassette sub family F member 1 antibody; ATP binding cassette, sub family F (GCN20), member 1 antibody; ATP-binding cassette 50 antibody; ATP-binding cassette sub-family F member 1 antibody; AU041969 antibody; D17Wsu166e antibody; EST123147 antibody; GCN20 antibody; TNF alpha stimulated ABC protein antibody; TNF-alpha-stimulated ABC protein antibody; TNFalpha inducible ATP binding protein antibody; wu:fb79c06 antibody; wu:fc39a05 antibody; wu:fj94a08 antibody; zgc:85667 antibody

SEQUENCE SIMILARITIES

Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

ABCF1 (ATP-binding cassette sub-family F member 1, TNF-alpha-stimulated ABC protein) is a 845 amino acid protein encoded by the human gene ABCF1. ABCF1 belongs to the ABC transporter family (EF3 subfamily) and contains two ABC transporter domains. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. ABCF1 is believed to have a role in mRNA translation due to its interaction with eukaryotic initiation factor 2 (eIF2). It is also associated with ribosomes. ABCF1 is ubiquitously expressed and can be induced with TNF. Upon DNA damage, ABCF1 is phosphorylated by either ATM or ATR.