PRODUCT CODE: ET1703-69

Recombinant A2M Monoclonal Antibody (ET1703-69)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution.
  • Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- A2M antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- A2M antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- A2M antibody. Counter stained with hematoxylin.
Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant A2M Monoclonal Antibody (ET1703-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta, human liver tissue, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM81-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

163 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Alpha-2-macroglobulin

GENE NAME

A2M

SYNONYMS

Alpha-2-M, A2M

SEQUENCE SIMILARITIES

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

TISSUE SPECIFICITY

Secreted in plasma.

DEVELOPMENTAL STAGE

Unlike the rat protein, which is an acute phase protein, this protein is always in circulation at high levels.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.