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PRODUCT CODE: ET1609-76

Recombinant Sodium Potassium ATPase Monoclonal Antibody (ET1609-76)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Sodium Potassium ATPase on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Sodium Potassium ATPase on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Sodium Potassium ATPase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Sodium Potassium ATPase in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Sodium Potassium ATPase was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Sodium Potassium ATPase on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Sodium Potassium ATPase Monoclonal Antibody (ET1609-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549 cell lysates, Hela, MCF-7, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST0533

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P05023

PROTEIN NAME

Sodium Potassium ATPase

SYNONYMS

ATPase Na+/K+ transporting alpha antibody; adenosinetriphosphatase antibody; AT1A1_HUMAN antibody; ATP1A1 antibody; ATP1A4 antibody; ATP1AL2 antibody; ATP1B antibody; ATP1B1 antibody; ATPase Na+/K+ transporting alpha 1 polypeptide antibody; ATPase Na+/K+ transporting alpha 4 polypeptide antibody; ATPase Na+/K+ transporting beta 1 polypeptide antibody; ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody; ATPase, Na+/K+ transporting, beta 1 polypeptide antibody; Beta 1-subunit of Na(+),K(+)-ATPase antibody; Na(+)/K(+) ATPase alpha-1 subunit antibody; Na(+)/K(+) ATPase alpha-4 subunit antibody; Na+, K+ ATPase alpha subunit antibody; Na+/K+ ATPase 1 antibody; Na+/K+ ATPase 4 antibody; Na+/K+ ATPase, alpha-D polypeptide antibody; Na, K-ATPase beta-1 polypeptide antibody; Na, K-ATPase, alpha-A catalytic polypeptide antibody; Na,K-ATPase catalytic subunit alpha-A protein antibody; Na,K-ATPase subunit alpha-C antibody; polypeptide-like 2 antibody; Sodium pump 1 antibody; sodium pump 4 antibody; Sodium pump subunit alpha-1 antibody; sodium pump subunit alpha-4 antibody; sodium pump subunit beta-1 antibody; sodium-potassium ATPase catalytic subunit alpha-1 antibody; sodium-potassium ATPase catalytic subunit alpha-4 antibody; sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody; sodium-potassium ATPase, alpha 4 polypeptide antibody; sodium-potassium-ATPase, alpha 1 polypeptide antibody; Sodium/potassium transporting ATPase alpha 1 chain antibody; Sodium/potassium transporting ATPase subunit beta 1 antibody; sodium/potassium-dependent ATPase beta-1 subunit antibody; sodium/potassium-transporting ATPase alpha-4 chain antibody; sodium/potassium-transporting ATPase beta-1 chain antibody; Sodium/potassium-transporting ATPase subunit alpha-1 antibody; sodium/potassium-transporting ATPase subunit alpha-4 antibody

SEQUENCE SIMILARITIES

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).

SUBCELLULAR LOCATION

Cell membrane, Melanosome.

FUNCTION

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.