PRODUCT CODE: ET7108-95

Recombinant Nectin 2 Monoclonal Antibody (ET7108-95)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.
  • Western blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.
  • ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Nectin 2 Monoclonal Antibody (ET7108-95)

Immunogen

Recombinant protein within human nectin 2 aa 50-250.

Host

Rabbit

Positive Control

K562, MCF-7, SK-OV-3, LOVO, rat testis tissue, human liver cancer tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG39-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Nectin 2

SYNONYMS

CD 112 antibody; CD112 antibody; CD112 antigen antibody; Herpes virus entry mediator B antibody; Herpes virus entry protein B antibody; Herpesvirus entry mediator B antibody; Herpesvirus entry protein B antibody; Hve B antibody; HveB antibody; Nectin-2 antibody; Nectin2 antibody; Poliovirus receptor like 2 antibody; Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody; Poliovirus receptor related 2 antibody; Poliovirus receptor related protein 2 antibody; Poliovirus receptor-related protein 2 antibody; PRR 2 antibody; PRR2 antibody; PVRL 2 antibody; PVRL2 antibody; PVRL2_HUMAN antibody; PVRR 2 antibody; PVRR2 antibody

SEQUENCE SIMILARITIES

Belongs to the nectin family.

TISSUE SPECIFICITY

Ubiquitous.

SUBCELLULAR LOCATION

Cell membrane. Membrane.

FUNCTION

Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human nectin is identical to the poliovirus receptor-related protein (PRR) and has been identified as the α-herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3; each member has two or three splicing variants. Nectin 2, also designated PRR2/HveB, is ubquitously expressed, with the highest levels of expression in some human neuronal cell lines, fibroblastic cells, keratinocytes and primary activated T lymphocytes. Nectin 2 has two splicing variants, Nectin 2α (short form) and 2δ (long form). Both Nectin 2α and 2δ have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein afadin, through which it is linked to the Actin cytoskeleton. The extracellular regions of the splicing variants are identical, but their transmembrane regions and cytoplasmic regions are unique. Nectin 2 mediates the entry of three mutant herpes simplex virus type 1 (HSV-1) strains that do not use HveA as co-receptor, but not wildtype HSV-1 strains. Nectin 2 also mediates the entry of HSV-2 and pseudorabies virus, but not bovine herpes virus type 1. Nectin 2δ is tyrosine phosphorylated in response to cell-cell adhesion.