PRODUCT CODE: ET7107-08

Recombinant GABA A Receptor alpha 5 Monoclonal Antibody (ET7107-08)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: A549 cell lysate <br />
 Lane 2: 293T cell lysate<br />
 Lane 3: Rat brain tissue lysate
  • Western blot analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: A549 cell lysate <br />
 Lane 2: 293T cell lysate<br />
 Lane 3: Rat brain tissue lysate
  • ICC staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.
Western blot analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: Rat brain tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant GABA A Receptor alpha 5 Monoclonal Antibody (ET7107-08)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

A549 cell lysate, 293T cell lysate, rat brain tissue lysate, SH-SY-5Y cell, 293T cell, A549 cell, rat brain tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB34-19

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GABA A Receptor alpha 5

SYNONYMS

GAA 5 antibody; GAA5 antibody; GABA(A) receptor subunit alpha-5 antibody; GABRA 5 antibody; Gabra5 antibody; Gamma aminobutyric acid GABA A receptor alpha 5 antibody; Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody; Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody; Gamma-aminobutyric acid receptor subunit alpha-5 antibody; GBRA5_HUMAN antibody; GC138184 antibody

SEQUENCE SIMILARITIES

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

Gamma-aminobutyric acid (GABA) A receptor, alpha 5, also known as GABRA5, is a protein which in humans is encoded by the GABRA5 gene. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABAA receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABAA receptor. At least 16 distinct subunits of GABAA receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. Recent research has produced several ligands which are moderately selective for GABAA receptors containing the α5 subunit. These have proved to be useful in investigating some of the side effects of benzodiazepine and nonbenzodiazepine drugs, particularly the effects on learning and memory such as anterograde amnesia. Inverse agonists at this subunit have nootropic effects and may be useful for the treatment of cognitive disorders such as Alzheimer's disease.