PRODUCT CODE: ET1702-76

Recombinant Ctip2 Monoclonal Antibody (ET1702-76)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Ctip2 on THP-1 cells lysates using anti-Ctip2 antibody at 1/1,000 dilution.
  • Western blot analysis of Ctip2 on THP-1 cells lysates using anti-Ctip2 antibody at 1/1,000 dilution.
  • ICC staining Ctip2 in LO2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Ctip2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Ctip2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Ctip2 on THP-1 cells lysates using anti-Ctip2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Ctip2 Monoclonal Antibody (ET1702-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, LO2, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF09-90

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100/110kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ctip2

SYNONYMS

ATL1 alpha antibody; ATL1 antibody; ATL1 beta antibody; ATL1 gamma antibody; ATL1-delta antibody; B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody; B cell lymphoma/leukemia 11B antibody; B-cell CLL/lymphoma 11B (zinc finger protein) antibody; B-cell CLL/lymphoma 11B antibody; B-cell lymphoma/leukemia 11B antibody; BC11B_HUMAN antibody; BCL-11B antibody; Bcl11b antibody; BCL11B/TRDC fusion antibody; COUP TF interacting protein 2 antibody; COUP-TF interacting protein 2 antibody; COUP-TF-interacting protein 2 antibody; Ctip 2 antibody; CTIP-2 antibody; CTIP2 antibody; hRIT1 alpha antibody; hRit1 antibody; Radiation induced tumor suppressor gene 1 antibody; Radiation induced tumor suppressor gene 1 protein antibody; Radiation-induced tumor suppressor gene 1 protein antibody; Rit 1 antibody; Rit1 antibody; zinc finger protein hRit1 alpha antibody; ZNF856B antibody

TISSUE SPECIFICITY

Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

POST-TRANSLATIONAL MODIFICATION

Sumoylated with SUMO1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Bcl-11a (CtIP-1, EVI9, B cell chronic lymphocytic leukemia (CLL)/lymphoma 11A) and Bcl-11b (CtIP-2, RIT1, B cell CLL/lymphoma 11B) genes play crucial roles in lymphopoiesis and influence the progression of hematopoietic malignancies. Disruption of the Bcl-11b locus is linked to T cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T cell lymphoma. Bcl-11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Bcl-11b can interact with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. Bcl-11a is essential for postnatal development and normal lymphopoiesis. The Bcl-11a mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with Bcl-6.