PRODUCT CODE: ET1702-62

Recombinant Annexin V Monoclonal Antibody (ET1702-62)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2 <br />
 Lane 3: JAR
  • Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2 <br />
 Lane 3: JAR
  • ICC staining Annexin V in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Annexin V in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with Annexin V antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: JAR

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Annexin V Monoclonal Antibody (ET1702-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, HepG2 cell lysate, Hela, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF50-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Annexin V

SYNONYMS

Anchorin CII antibody; Annexin 5 antibody; Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody; ANX A5 antibody; ANX5 antibody; ANXA5 antibody; ANXA5_HUMAN antibody; Calphobindin I antibody; CBP-I antibody; Endonexin II antibody; ENX2 antibody; Lipocortin V antibody; PAP I antibody; PAP-I antibody; Placental anticoagulant protein 4 antibody; Placental anticoagulant protein I antibody; PLACENTAL PROTEIN 4 antibody; Pp4 antibody; Thromboplastin inhibitor antibody; VAC-alpha antibody; Vascular anticoagulant-alpha antibody

SEQUENCE SIMILARITIES

Belongs to the annexin family.

POST-TRANSLATIONAL MODIFICATION

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.

FUNCTION

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation.