Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: JAR
Applications
-
WB
-
ICC
-
IF
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Annexin V Monoclonal Antibody (ET1702-62)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, Hela, A431.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF50-11
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
36 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
FC
-
1:50-1:100
-
ICC/IF
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Annexin V
SYNONYMS
Anchorin CII antibody; Annexin 5 antibody; Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody; ANX A5 antibody; ANX5 antibody; ANXA5 antibody; ANXA5_HUMAN antibody; Calphobindin I antibody; CBP-I antibody; Endonexin II antibody; ENX2 antibody; Lipocortin V antibody; PAP I antibody; PAP-I antibody; Placental anticoagulant protein 4 antibody; Placental anticoagulant protein I antibody; PLACENTAL PROTEIN 4 antibody; Pp4 antibody; Thromboplastin inhibitor antibody; VAC-alpha antibody; Vascular anticoagulant-alpha antibody
SEQUENCE SIMILARITIES
Belongs to the annexin family.
POST-TRANSLATIONAL MODIFICATION
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
SUBCELLULAR LOCATION
Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.
FUNCTION
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation.