PRODUCT CODE: ET1704-87

Recombinant 5HT2C Receptor Monoclonal Antibody (ET1704-87)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 5HT2C Receptor on A431 (1) AND K562 (2) lysates using anti-5HT2C Receptor antibody at 1/1,000 dilution.
  • Western blot analysis of 5HT2C Receptor on A431 (1) AND K562 (2) lysates using anti-5HT2C Receptor antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-5HT2C Receptor antibody. Counter stained with hematoxylin.
Western blot analysis of 5HT2C Receptor on A431 (1) AND K562 (2) lysates using anti-5HT2C Receptor antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 5HT2C Receptor Monoclonal Antibody (ET1704-87)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, K562, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA31-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

5-hydroxytryptamine receptor 2C

GENE NAME

HTR2C

SYNONYMS

5-HT-2C, 5-HT2C, 5-HTR2C, 5-HT-1C, 5-HT1C, HTR2C, HTR1C

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in brain.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.