Lane 1: F9 cell lysate
Lane 2: HES cell lysate
Recombinant Rabbit monoclonal primary
Recombinant 44108 Monoclonal Antibody (ET1612-20)
F9, NCCIT, hES, N2A, MCF-7, mouse liver tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
POU domain, class 5, transcription factor 1
Oct-4, Oct-3, OTF-3, POU5F1, OCT3, OCT4, OTF3
Belongs to the POU transcription factor family. Class-5 subfamily.
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.; Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Bin, Gu et al.
Aire promotes the self-renewal of embryonic stem cells through Lin28. | Stem Cells and Development 
Wang, Yongfang et al.
N-myc downstream regulated gene 1(NDRG1) promotes the stem-like properties of lung cancer cells through stabilized c-Myc. | Cancer Letters