Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Applications
-
WB
-
ICC
-
IF
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant 14-3-3 Theta Monoclonal Antibody (ET1612-97)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
MCF-7 cell lysate, Hela cell lysate, Hela, A431, A549, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD084-02
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
28 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
FC
-
1:50-1:100
-
ICC/IF
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
14-3-3 Theta
SYNONYMS
14-3-3 antibody; 14-3-3 protein T-cell antibody; 14-3-3 protein tau antibody; 14-3-3 protein theta antibody; 1C5 antibody; HS1 antibody; theta polypeptide antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody
SEQUENCE SIMILARITIES
Belongs to the 14-3-3 family.
TISSUE SPECIFICITY
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
POST-TRANSLATIONAL MODIFICATION
Ser-232 is probably phosphorylated by CK1.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.