Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Recombinant Rabbit monoclonal primary
Recombinant 14-3-3 Theta Monoclonal Antibody (ET1612-97)
MCF-7 cell lysate, Hela cell lysate, Hela, A431, A549, SH-SY5Y.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
14-3-3 antibody; 14-3-3 protein T-cell antibody; 14-3-3 protein tau antibody; 14-3-3 protein theta antibody; 1C5 antibody; HS1 antibody; theta polypeptide antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody
Belongs to the 14-3-3 family.
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
Ser-232 is probably phosphorylated by CK1.
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.