PRODUCT CODE: ET1612-41

Recombinant 14-3-3 sigma Monoclonal Antibody (ET1612-41)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 sigma Monoclonal Antibody (ET1612-41)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human tonsil tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2070

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 sigma

SYNONYMS

14 3 3 protein sigma antibody; 14-3-3 protein sigma antibody; 1433S_HUMAN antibody; Epithelial cell marker protein 1 antibody; Er antibody; HME 1 antibody; HME1 antibody; MGC143283 antibody; Mkrn3 antibody; Mme1 antibody; OTTHUMP00000004242 antibody; RP23 137L22.11 antibody; SFN antibody; SFN protein antibody; Stratifin antibody; YWHAS antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

TISSUE SPECIFICITY

Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Secreted.

FUNCTION

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.