PRODUCT CODE: ET1701-4

Recombinant 14-3-3 Monoclonal Antibody (ET1701-4)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HT29 <br />
 Lane 2: Human skin <br />
 Lane 3: NIH/3T3
  • Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HT29 <br />
 Lane 2: Human skin <br />
 Lane 3: NIH/3T3
Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HT29
Lane 2: Human skin
Lane 3: NIH/3T3

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 Monoclonal Antibody (ET1701-4)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, HT29, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ084-3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28/19 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 protein beta/alpha

GENE NAME

YWHAB

SYNONYMS

KCIP-1, YWHAB, YWHAZ

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

SUBCELLULAR LOCATION

Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane. Note=(Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG.

FUNCTION

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.