PRODUCT CODE: ET1701-2

Recombinant 14-3-3 Monoclonal Antibody (ET1701-2)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: HT29 <br />
  Lane 2: Human skin <br />
  Lane 3: NIH/3T3
  • Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: HT29 <br />
  Lane 2: Human skin <br />
  Lane 3: NIH/3T3
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HT29
Lane 2: Human skin
Lane 3: NIH/3T3

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 Monoclonal Antibody (ET1701-2)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, HT29, human skin tissue, mouse skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ083-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

14-3-3

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

SUBCELLULAR LOCATION

Cytoplasm, Melanosome.

FUNCTION

The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.