PRODUCT CODE: ET1612-9

Recombinant 14-3-3 gamma Monoclonal Antibody (ET1612-9)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: A431 <br />
 Lane 3: Hela
  • Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: A431 <br />
 Lane 3: Hela
  • Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: A431
Lane 3: Hela

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 gamma Monoclonal Antibody (ET1612-9)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD20-65

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 gamma

SYNONYMS

14 3 3 gamma antibody; 14 3 3 protein gamma antibody; 14 3 3 protein gamma subtype antibody; 14 3 3gamma antibody; 14-3-3 protein gamma antibody; 1433G_HUMAN antibody; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody; KCIP 1 antibody; KCIP-1 antibody; KCIP1 antibody; N-terminally processed antibody; Protein kinase C inhibitor protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide antibody; Ywhag antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

TISSUE SPECIFICITY

Highly expressed in brain, skeletal muscle, and heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by various PKC isozymes.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.