PRODUCT CODE: ET1701-1

Recombinant 14-3-3 epsilon Monoclonal Antibody (ET1701-1)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: SH-SY-5Y <br />
Lane 2: 293T
  • Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: SH-SY-5Y <br />
Lane 2: 293T
  • ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 epsilon in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 epsilon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 epsilon antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: 293T

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 epsilon Monoclonal Antibody (ET1701-1)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY-5Y, NIH/3T3, Hela, 293T, human colon cancer tissue, mouse placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-40

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:10-1:100

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 protein epsilon

GENE NAME

YWHAE

SYNONYMS

14-3-3E, YWHAE

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

FUNCTION

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.