PRODUCT CODE: ET1612-99

Recombinant 14-3-3 alpha+beta Monoclonal Antibody (ET1612-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: 293T <br />
 Lane 3: HepG2
  • Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: 293T <br />
 Lane 3: HepG2
  • ICC staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 alpha+beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: HepG2

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant 14-3-3 alpha+beta Monoclonal Antibody (ET1612-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, HepG2 cell lysate, 293T cell lysate, Hela, A431, SHG-44, human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0837

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 alpha+beta

SYNONYMS

14 3 3 alpha antibody; 14 3 3 protein beta/alpha antibody; 14-3-3 protein beta/alpha antibody; 1433B_HUMAN antibody; Brain protein 14 3 3 beta isoform antibody; GW128 antibody; HS 1 antibody; KCIP-1 antibody; KCIP1 antibody; N-terminally processed antibody; Protein 1054 antibody; Protein kinase C inhibitor protein 1 antibody; YWHAA antibody; YWHAB antibody

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

SUBCELLULAR LOCATION

Cytoplasm, Melanosome.

FUNCTION

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.