Lane 1: Hela
Lane 2: 293T
Lane 3: HepG2
Recombinant Rabbit monoclonal primary
Recombinant 14-3-3 alpha+beta Monoclonal Antibody (ET1612-99)
Hela cell lysate, HepG2 cell lysate, 293T cell lysate, Hela, A431, SHG-44, human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
14 3 3 alpha antibody; 14 3 3 protein beta/alpha antibody; 14-3-3 protein beta/alpha antibody; 1433B_HUMAN antibody; Brain protein 14 3 3 beta isoform antibody; GW128 antibody; HS 1 antibody; KCIP-1 antibody; KCIP1 antibody; N-terminally processed antibody; Protein 1054 antibody; Protein kinase C inhibitor protein 1 antibody; YWHAA antibody; YWHAB antibody
Belongs to the 14-3-3 family.
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.