PRODUCT CODE: ER1802-9

RBX1 Antibody (ER1802-9)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.
  • Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.
  • ICC staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RBX1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with RBX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

RBX1 Antibody (ER1802-9)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, 293T, MCF-7, human colon cancer tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RBX1

SYNONYMS

BA554C12.1 antibody; E3 ubiquitin-protein ligase RBX1 antibody; FLJ60363 antibody; MGC13357 antibody; MGC1481 antibody; OTTHUMP00000028983 antibody; Protein ZYP antibody; Rbx 1 antibody; Rbx1 antibody; RBX1_HUMAN antibody; Regulator of cullins 1 antibody; Ring box 1 antibody; Ring box 1 E3 ubiquitin protein ligase antibody; RING box protein 1 antibody; RING finger protein 75 antibody; RING finger protein antibody; RING-box protein 1 antibody; Ringbox protein 1 antibody; RNF 75 antibody; RNF75 antibody; ROC 1 antibody; ZYP protein antibody

SEQUENCE SIMILARITIES

Belongs to the RING-box family.

TISSUE SPECIFICITY

Widely expressed.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Rbx1 (also designated ROC1 and Hrt1) and the closely related protein Rbx2 (also designated ROC2) are RING finger containing homologs of the yeast protein APC11, a member of the anaphase-promoting complex (APC). Rbx1 was shown to be a component of the von Hippel-Lindau (VHL) transcription elongation complex, which includes VHL, Elongin B, Elongin C and Cullin-2. Rbx1 interacts with Cullin-1 in the SCF (Skp1-Cdc53-F-box protein) ubiquitin ligase complex (2-4). Rbx1 functions as a common subunit of SCF complexes required for ubiquination of various proteins including yeast G1 cyclins, IkBa and b-catenin (2-4). Rbx1 was shown to enhance the ubiquitin ligase activity of the VHL/Cullin-2 complex, and of the SCF/Cullin-1 complex.