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RBM3 Recombinant Rabbit Monoclonal Antibody [JE63-84]

Usd: 385

Specification

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Catalog# HA721130

RBM3 Recombinant Rabbit Monoclonal Antibody [JE63-84]

Application

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Mouse

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RBM3 Recombinant Rabbit Monoclonal Antibody [JE63-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RBM3 aa 58-157/157.

Species Reactivity

Mouse, Human, Rat

Validated Applications

IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 17 kDa

Positive Control

Hela, human esophagus tissue, mouse thyroid tissue, mouse trachea tissue, rat trachea tissue, rat esophagus tissue, THP-1.

Conjugation

unconjugated

Clone Number

JE63-84

RRID

AB_3072254

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:400

  • FC

  • 1:500-1:1,000

Target

Function

Putative RNA-binding protein 3 is a protein that in humans is encoded by the RBM3 gene. This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RBM3 is cold-induced RNA binding protein and is involved in mRNA biogenesis exerts anti-apoptotic effects. According to antibody-based profiling and transcriptomics analysis, RBM3 protein is present in all analysed human tissues and based on confocal microscopy mainly localised to the nucleoplasm. RBM3 is a proto-oncogene that is associated with tumor progression and metastasis and is a potential cancer biomarker. Based on patient survival data, high levels of RBM3 protein in tumor cells is a favourable prognostic biomarker in colorectal cancer.

Background References

1. Miao X. et. al. Role of RBM3 in the regulation of cell proliferation in hepatocellular carcinoma. Exp Mol Pathol. 2020 Dec

2. Jackson TC. et. al. Robust RBM3 and beta-klotho expression in developing neurons in the human brain. J Cereb Blood Flow Metab. 2019 Dec

Subcellular Location

Nucleus, cytoplasm, dendrite.

UNIPROT

SwissProt: P98179 Human

SwissProt: O89086 Mouse

SwissProt: Q925G0 Rat

Synonyms

2600016C11Rik antibody

IS1 RNPL antibody

MGC105811 antibody

MGC118410 antibody

OTTHUMP00000025800 antibody

OTTHUMP00000025802 antibody

OTTMUSP00000019634 antibody

OTTMUSP00000019635 antibody

OTTMUSP00000019636 antibody

Putative RNA binding protein 3 antibody

Expand

Images

  • Immunocytochemistry analysis of Hela cells labeling RBM3 with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta Ⅲ tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor&reg; 647) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Hela cells labeling RBM3 with Rabbit anti-RBM3 antibody (HA721130) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RBM3 antibody (HA721130) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta Ⅲ tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-RBM3 antibody (HA721130) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721130) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Rabbit anti-RBM3 antibody (HA721130) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721130) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-RBM3 antibody (HA721130) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721130) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat trachea tissue with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat trachea tissue with Rabbit anti-RBM3 antibody (HA721130) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721130) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-RBM3 antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-RBM3 antibody (HA721130) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721130) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of THP-1 cells labeling RBM3.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721130" style="font-weight: bold;text-decoration: underline;">HA721130</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling RBM3.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721130, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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