PRODUCT CODE: ER40115

Ras Antibody (ER40115)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: PC12 <br />
Lane 2: MCF-7 <br />
Lane 3: 293T <br />
Lane 4: Mouse brain <br />
Lane 5: Mouse intestine <br />
Lane 6: Rat brain
  • Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: PC12 <br />
Lane 2: MCF-7 <br />
Lane 3: 293T <br />
Lane 4: Mouse brain <br />
Lane 5: Mouse intestine <br />
Lane 6: Rat brain
  • ICC staining Ras in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ras in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ras in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ras in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ras antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney muscle tissue using anti-Ras antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.
Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution.
Positive control:
Lane 1: PC12
Lane 2: MCF-7
Lane 3: 293T
Lane 4: Mouse brain
Lane 5: Mouse intestine
Lane 6: Rat brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Ras Antibody (ER40115)

Immunogen

Peptide

Host

Rabbit

Positive Control

PC12, MCF-7, 293T, Hela, HepG2, mouse brain tissue, mouse intestines tissue, rat brain tissue, human kidney tissue, mouse kidney tissue, mouse small intestine tissue, mouse pancreas tissue, rat kidney tissue, rat small intestine tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

GTPase NRas

GENE NAME

NRAS

SYNONYMS

Ras

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Ras family.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.; Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).; Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.; Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.

SUBCELLULAR LOCATION

Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.

FUNCTION

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

CITATIONS

  • Qiu, Qiang et al.

    Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma. | Oncotarget [2019]

  • Shen, Junyi et al.

    Loss of FoxA2 accelerates neoplastic changes in the intrahepatic bile duct partly via the MAPK signaling pathway. | Aging [2019]