Mouse monoclonal primary
RAP80 Monoclonal Antibody (EM1902-05)
Recombinant protein within human rap80 aa 300-550.
Siha cell lysates, rat testis tissue, mouse testis tissue, SiHa cell.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
BRCA1-A complex subunit RAP80
Belongs to the RAP80 family.
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Sumoylated.; Phosphorylated upon DNA damage by ATM or ATR.
Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs).
Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.