Lane 1: SKBR-3 cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Rat brain tissue lysate
Rabbit polyclonal primary
RanBP9 Antibody (ER2001-63)
Recombinant protein within human ranbp9 aa 800-1041.
SKBR-3 cell lysate, 293 cell lysate, mouse lung tissue lysate, rat brain tissue lysate, F9.
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein affinity purified.
Jaekel S. et. al. Importins fulfill a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains. EMBO J. 21:377-386(2002).
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS7, RPL18A, RPL6, histone H2A, histone H2B and histone. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. Mediates the nuclear import of actin .