Positive control:
Lane 1: SKBR-3 cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Rat brain tissue lysate
Applications
-
WB
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
RanBP9 Antibody (ER2001-63)
Immunogen
Recombinant protein within human ranbp9 aa 800-1041.
Host
Rabbit
Positive Control
SKBR-3 cell lysate, 293 cell lysate, mouse lung tissue lysate, rat brain tissue lysate, F9.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
116 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
RanBP9
SYNONYMS
Imp9 antibody; Imp9a antibody; Imp9b antibody; Importin-9 antibody; Importin-9a antibody; Ipo9 antibody; IPO9_HUMAN antibody; Ran-binding protein 9 antibody; RanBP9 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS7, RPL18A, RPL6, histone H2A, histone H2B and histone. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. Mediates the nuclear import of actin .