PRODUCT CODE: ER1802-61

Rae1 Antibody (ER1802-61)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3<br />
 Lane 2: Mouse testis tissue<br />
 Lane 3: Rat testis tissue<br />
  • Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3<br />
 Lane 2: Mouse testis tissue<br />
 Lane 3: Rat testis tissue<br />
  • ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3
Lane 2: Mouse testis tissue
Lane 3: Rat testis tissue

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Rae1 Antibody (ER1802-61)

Immunogen

Synthetic peptide within human rae1 aa 330-368.

Host

Rabbit

Positive Control

PC-3, mouse testis tissue, rat testis tissue, LOVO, MCF-7, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

mRNA export factor

GENE NAME

RAE1

SYNONYMS

RAE1, MRNP41

SEQUENCE SIMILARITIES

Belongs to the WD repeat rae1 family.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Recruited from interphase nuclei to spindle MTs during mitosis.

FUNCTION

Plays a role in mitotic bipolar spindle formation. Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.