PRODUCT CODE: ER1802-53

RAB7 Antibody (ER1802-53)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of RAB7 on MCF-7 cell lysate using anti-RAB7 antibody at 1/500 dilution.
  • Western blot analysis of RAB7 on MCF-7 cell lysate using anti-RAB7 antibody at 1/500 dilution.
  • ICC staining RAB7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RAB7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RAB7 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with RAB7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of RAB7 on MCF-7 cell lysate using anti-RAB7 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

RAB7 Antibody (ER1802-53)

Immunogen

Full length recombinant protein of human rab7.

Host

Rabbit

Positive Control

MCF-7, A431, SiHa, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RAB7

SYNONYMS

CMT2B antibody; PRO2706 antibody; PSN antibody; RAB7, member RAS oncogene family antibody; RAB7A antibody; RAB7A, member RAS oncogene family antibody; RAB7A_HUMAN antibody; Ras associated protein RAB7 antibody; Ras related protein Rab7 antibody; Ras related protein Rab7a antibody; Ras-related protein Rab-7a antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rab family.

TISSUE SPECIFICITY

Widely expressed; high expression found in skeletal muscle.

SUBCELLULAR LOCATION

Endosome. Lysosome.

FUNCTION

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.