PRODUCT CODE: ER31215

PUMA Antibody (ER31215)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse kidney
  • Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse kidney
  • ICC staining PUMA in SKOV-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PUMA in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PUMA in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with PUMA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PUMA Antibody (ER31215)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, mouse kidney tissue, SKOV-3,Lovo, Jurkat, human breast cancer tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC:1:100

TARGET

UNIPROT #

PROTEIN NAME

Bcl-2-binding component 3, isoforms 1/2

GENE NAME

BBC3

SYNONYMS

PUMA,BBC3

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Ubiquitously expressed.

SUBCELLULAR LOCATION

Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release.

FUNCTION

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.