PRODUCT CODE: ER1802-35

PTP1B Antibody (ER1802-35)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution.
  • Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution.
  • ICC staining PTP1B in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTP1B in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PTP1B Antibody (ER1802-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Recombinant protein lysate, LOVO, SiHa, human colon cancer tissue, human placenta tissue, mouse fallopian tubes tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1

  • 5,000

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

PTP1B

SYNONYMS

PTP1B antibody; Non receptor tyrosine phosphatase 1 antibody; Protein phosphotyrosylphosphatase 1B antibody; Protein tyrosine phosphatase 1B antibody; Protein tyrosine phosphatase non receptor type 1 antibody; Protein tyrosine phosphatase placental antibody; Protein-tyrosine phosphatase 1B antibody; PTN1_HUMAN antibody; PTP 1B antibody; PTP-1B antibody; PTPN 1 antibody; PTPN1 antibody; Tyrosine protein phosphatase non receptor type 1 antibody; Tyrosine-protein phosphatase non-receptor type 1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

TISSUE SPECIFICITY

Expressed in keratinocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.