PRODUCT CODE: M1701-7

PTBP1 Monoclonal Antibody (M1701-7)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of PTBP1 on different cell lysate using anti-PTBP1 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Daudi Lane 2: Hela Lane 3: PC-12
  • Western blot analysis of PTBP1 on different cell lysate using anti-PTBP1 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Daudi Lane 2: Hela Lane 3: PC-12
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Western blot analysis of PTBP1 on different cell lysate using anti-PTBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Daudi Lane 2: Hela Lane 3: PC-12

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PTBP1 Monoclonal Antibody (M1701-7)

Immunogen

Peptide

Host

Mouse

Positive Control

Daudi, Hela, PC-12, human tonsil tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5C1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

57 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PTBP1

SYNONYMS

57 kDa RNA binding protein PPTB 1 antibody; 57 kDa RNA-binding protein PPTB-1 antibody; Heterogeneous nuclear ribonucleoprotein I antibody; Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody; hnRNP I antibody; HNRNP-I antibody; HNRNPI antibody; HNRPI antibody; MGC10830 antibody; MGC8461 antibody; Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody; Polypyrimidine Tract Binding Protein 1 antibody; Polypyrimidine tract binding protein antibody; Polypyrimidine tract-binding protein 1 antibody; pPTB antibody; PTB 1 antibody; PTB 2 antibody; PTB 3 antibody; PTB 4 antibody; PTB antibody; PTB T antibody; PTB1 antibody; PTB2 antibody; PTB3 antibody; PTB4 antibody; PTBP 1 antibody; PTBP1 antibody; PTBP1_HUMAN antibody; PTBT antibody; RNA Binding Protein antibody

SUBCELLULAR LOCATION

Nucleus. 

FUNCTION

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB), and its homolog hnRNP L bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a protein that is localized in the nucleoplasm. hnRNP L, like hnRNP I, is also localized in the nucleoplasm.