PRODUCT CODE: em1701-63

PTBP1 Monoclonal Antibody (EM1701-63)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution.<br />
 Positive control:<br />
 Lane 1: Jurkat <br />
 Lane 2: A431<br />
 Lane 3: K562<br />
  • Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution.<br />
 Positive control:<br />
 Lane 1: Jurkat <br />
 Lane 2: A431<br />
 Lane 3: K562<br />
  • ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with PTBP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A431
Lane 3: K562

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PTBP1 Monoclonal Antibody (EM1701-63)

Immunogen

Recombinant protein within human ptbp1 aa 320-550.

Host

Mouse

Positive Control

Jurkat, A431, K562, rat testis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, 293T, A549, SW480, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9A1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • IHC-P

  • 1:50-1:100

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Polypyrimidine tract-binding protein 1

GENE NAME

PTBP1

SYNONYMS

PTB, hnRNP I, PTBP1, PTB

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.