PRODUCT CODE: ER1706-25

PRTN3 Antibody (ER1706-25)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.
  • Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.
  • Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution.
  • ICC staining PRTN3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PRTN3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PRTN3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PRTN3 antibody. Counter stained with hematoxylin.
Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PRTN3 Antibody (ER1706-25)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

U937, mouse marrow lysate, 293T, A549, SH-SY5Y, human lung cancer tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

PRTN3

SYNONYMS

ACPA antibody; AGP 7 antibody; AGP7 antibody; AGP7 serine proteinase antibody; Azurophil Granule Protein 7 antibody; C ANCA antibody; C ANCA antigen antibody; C-ANCA antigen antibody; CANCA antibody; EC 3.4.21.76 antibody; Leukocyte proteinase 3 antibody; MBN antibody; MBT antibody; MBT WEGENER AUTOANTIGEN antibody; Myeloblastin antibody; Neutrophil proteinase 4 antibody; NP 4 antibody; NP-4 antibody; NP4 antibody; P29 antibody; PR 3 antibody; PR-3 antibody; PR3 antibody; Proteinase 3 antibody; Proteinase3 antibody; PRTN 3 antibody; Prtn3 antibody; PRTN3_HUMAN antibody; Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody; Serine proteinase, neutrophil antibody; Wegener autoantigen antibody; Wegener granulomatosis autoantigen antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family. Elastase subfamily.

TISSUE SPECIFICITY

Expressed in polymorphonuclear leukocytes (at protein level). Expressed in neutrophils (at protein level). Expressed in differentiating neutrophils.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The major features of Wegener granulomatosis are necrotizing granulomatous lesions, which most often affect the upper and lower airways and are associated with vasculitis, necrotizing glomerulonephritis and pulmonary capillaritis. The antigen responsible for this disease is Proteinase 3 (PR3, P29 or myeloblastin), which is one of the antibiotic proteins of neutrophilic granules belonging to the serine protease family. It is closely related to two others: Neutrophil Elastase and azurocidin. All three genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. PR3 is a neutrophil protein which is able to cleave elastin and is involved in proliferation of human leukemia cells. PR3 is expressed specifically in immature myeloid cells and is a G-CSF-responsive protein critical to factor-independent growth. The genes for all three of the related serine protease family members are located in a cluster on the tip of the short arm of human chromosome 19.