PRODUCT CODE: ET1612-28

pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57] (ET1612-28)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of pro Caspase 7 on human lung lysates using anti-pro Caspase 7 antibody at 1/1,000 dilution.
  • Western blot analysis of pro Caspase 7 on human lung lysates using anti-pro Caspase 7 antibody at 1/1,000 dilution.
  • ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining pro Caspase 7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.
Western blot analysis of pro Caspase 7 on human lung lysates using anti-pro Caspase 7 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

pro Caspase-7 Recombinant Rabbit Monoclonal Antibody [SD20-57] (ET1612-28)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, human liver tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD20-57

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

34 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

pro Caspase-7

SYNONYMS

apoptosis-related cysteine peptidase antibody; Apoptotic protease Mch-3 antibody; CASP-7 antibody; CASP7 antibody; CASP7_HUMAN antibody; Caspase-7 subunit p11 antibody; Caspase-7 antibody; Caspase 7 antibody; Caspase7 antibody; CMH-1 antibody; ICE-LAP3 antibody; ICE-like apoptotic protease 3 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

POST-TRANSLATIONAL MODIFICATION

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.