PRODUCT CODE: em1701-72

PRDX2 Monoclonal Antibody (EM1701-72)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti-Peroxiredoxin 2 antibody at 1/5,000 dilution.
  • Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti-Peroxiredoxin 2 antibody at 1/5,000 dilution.
  • Flow cytometric analysis of PC-3M cells with Peroxiredoxin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti-Peroxiredoxin 2 antibody at 1/5,000 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PRDX2 Monoclonal Antibody (EM1701-72)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

PC-3M, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7F5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Peroxiredoxin-2

GENE NAME

PRDX2

SYNONYMS

NKEF-B, TSA, PRDX2, NKEFB, TDPX1

SEQUENCE SIMILARITIES

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

POST-TRANSLATIONAL MODIFICATION

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).